1. Mwachidule

Onani ziwerengero zotsatirazi mukuwerenga mutuwu.

1.1 Chiyambi Chachikulu

1.1.1 Miyezo

TS EN ISO/DIS 22611 Zovala zodzitchinjiriza kumatenda opatsirana - Njira yoyesera ya kukana kulowa mkati ndi ma aerosol oipitsidwa ndi biologically.

1.1.2 Zofotokozera

lJenereta ya aerosol:    Atomizer

lChipinda chowonetsera:  Mtengo PMMA

lKusonkhana kwachitsanzo :2, chitsulo chosapanga dzimbiri

lpompa pampu:Mpaka 80kpa

lDimension: 300mm*300mm*300mm

lMagetsi  :220V 50-60Hz

L Makina Kukula: 46cm×93cm×49cm (H)

l Kulemera Kwambiri: 35kg

2. KUGWIRITSA NTCHITO Zipangizo

2.1 Kukonzekera

Ikani magawo atatu mu kabati ya biosafety.Yang'anani mbali iliyonse ya makina oyesera ndikuonetsetsa kuti mbali zonse zikugwira ntchito bwino ndikugwirizanitsa bwino.

Kudula zitsanzo zisanu ndi zitatu monga 25mm m'mimba mwake.

Konzani chikhalidwe chausiku cha Staphylococcus aureus mwa kusamutsa baseptic kwa bakiteriya kuchokera ku michere ya agar (yosungidwa pa 4 ± 1 ℃) kupita ku msuzi wopatsa thanzi ndikuyika pa 37±1℃ pa shaker ya orbital.

Sungunulani chikhalidwecho kukhala mulingo woyenera wa mchere wosabala wa isotonic kuti mupereke chiwerengero chomaliza cha mabakiteriya pafupifupi 5*10.⁷ma cell cm^-3pogwiritsa ntchito chipinda chowerengera mabakiteriya a Thoma.

Lembani chikhalidwe pamwambapa mu atomizer.Mulingo wamadzimadzi uli pakati pa mlingo wapamwamba ndi wotsika.

2.2 Ntchito

Ikani chitsanzo cha msonkhano.Ikani silicone washer A, nsalu yoyesera, silicone washer B, nembanemba, waya wothandizira pachivundikiro chotseguka, kuphimba ndi maziko.

Ikani chitsanzo china cha msonkhano popanda chitsanzo.

Tsegulani chivindikiro chapamwamba cha chipinda choyesera.

Ikani chitsanzo cha msonkhano ndi chitsanzo ndi msonkhano popanda chitsanzo ndi Fasten of Fig. 4-1.

Onetsetsani kuti machubu onse alumikizidwa bwino.

Lumikizani mpweya woponderezedwa kuti musinthe mpweya.

Ikani mpweya pakuyenda kwa 5L / min posintha mita yoyenda ku atomizer ndikuyamba kupanga aerosol.

Pambuyo pa mphindi zitatu yambitsani pampu ya vacumm.Ikani 70kpa.

Pambuyo pa mphindi zitatu, zimitsani mpweya wa atomizer, koma siyani pampu ya vacuum ikuyenda kwa 1 min.

Zimitsani pampu yotsekera.

Chotsani zitsanzo za misonkhano mu chipinda.Ndipo sinthani mozama ma nembanemba a 0.45um ku mabotolo achilengedwe onse okhala ndi 10ml wosabala isotonic saline.

Chotsani pogwedeza kwa 1 min.Ndipo pangani ma serial dilutions ndi saline wosabala.(10^-1, 10^-2, 10^-3,ndi 10^-4)

Dulani ma aliquot 1ml a dilution iliyonse mobwerezabwereza pogwiritsa ntchito michere ya agar.

Ikani mbale usiku wonse pa 37 ± 1 ℃ ndikuwonetsa zotsatira zake pogwiritsa ntchito chiŵerengero cha mabakiteriya akumbuyo ku chiwerengero cha mabakiteriya omwe adadutsa poyesa chitsanzo.

Pangani ziganizo zinayi pamtundu uliwonse wa nsalu kapena chikhalidwe cha nsalu.

3. KUKONZA

Mofanana ndi zida zonse zamagetsi, chipangizochi chiyenera kugwiritsidwa ntchito moyenera ndipo kukonza ndi kuyang'anira kuyenera kuchitika pafupipafupi.Kusamala kotereku kudzatsimikizira kugwira ntchito kotetezeka komanso koyenera kwa zida.

Kusamalira nthawi ndi nthawi kumakhala ndi zowunikira zomwe zimachitidwa mwachindunji ndi woyesa komanso/kapena ndi ogwira ntchito ovomerezeka.

Kukonza zida ndi udindo wa wogula ndipo kuyenera kuchitidwa monga momwe tafotokozera mutuwu.

Kulephera kuchita zomwe akulimbikitsidwa kukonza kapena kukonza kochitidwa ndi anthu osaloledwa kungathe kulepheretsa chitsimikizocho.

1. Makinawo ayenera kuyang'aniridwa kuti apewe kutayikira kwa maulumikizidwe asanayesedwe;

2. Kusuntha makina ndikoletsedwa mukamagwiritsa ntchito;

3. Sankhani mphamvu yofananira ndi magetsi.Osakwera kwambiri kuti apewe kuwotcha chipangizo;

4. Chonde titumizireni kuti tigwire mu nthawi pamene makina sali bwino;

5. Iyenera kukhala ndi malo abwino opumira mpweya pamene makina amagwira ntchito;

6. Kuyeretsa makina pambuyo pa mayeso nthawi iliyonse;