1. Muchidimbu

Tarisa kunhamba dzinotevera uchiverenga chitsauko chino.

1.1 Nhanganyaya Huru

1.1.1 Mitemo

TS EN ISO/DIS 22611 Chipfeko chekudzivirira kubva kune chinotapukira-Nzira yekuyedza yekudzivirira kupinda neiyo biologically yakasvibiswa aerosols.

1.1.2 Zvinotsanangurwa

lAerosol jenareta:    Atomizer

lExposure chamber:  PMMA

lSample musangano :2, simbi isina tsvina

lVacumm pombi:Kusvika ku80kpa

lDimension: 300mm*300mm*300mm

lMagetsi  :220V 50-60Hz

l Machine Dimension: 46cm×93cm×49cm (H)

l Huremu hweNet: 35kg

2. KUSHANDISWA KWEZVINHU

2.1 Kugadzirira

Isa zvikamu zvitatu mubhabhiyosafety cabinet.Tarisa chikamu chimwe nechimwe chemuchina wekuyedza uye ita shuwa kuti zvikamu zvese zvinoshanda zvakanaka uye zvichibatana zvakanaka.

Kucheka sampuli sere se 25mm dhayamita madenderedzwa.

Gadzirira tsika yehusiku hweStaphylococcus aureus neaseptic kutapurirana kwebhakitiriya kubva kune zvinovaka muviri agar (yakachengetwa pa4±1℃) muhukasha muto uye incubation pa37±1℃ pane orbital shaker.

Dhonza tsika muhuwandu hwakakodzera hweisina isotonic saline kupa huwandu hwekupedzisira hwebhakitiriya hunosvika 5 * 10.⁷maseru cm^-3uchishandisa Thoma bacterial counting chamber.

Zadza tsika iri pamusoro muatomizer.Liquid level iri pakati pepamusoro nepasi.

2.2 Kushanda

Isa muenzaniso wegungano.Isa silicone washer A, bvunzo jira, silicone washer B, membrane, waya tsigiro pachivharo chakashama, vhara nechigadziko.

Isa imwe yemuenzaniso musangano pasina sampuli.

Vhura chivharo chepamusoro chekamuri yekuyedza.

Isa gungano remuenzaniso nemuenzaniso uye gungano pasina sampuli neFasten yeFig. 4-1.

Ita shuwa kuti machubhu ese akabatana zvakanaka.

Batanidza mweya wakadzvanywa kune wakadzvanywa mweya kugadzirisa.

Isa mhepo pakuyerera kwe5L/min nekugadzirisa kuyerera kwemamita kune atomizer uye tanga kugadzira iyo aerosol.

Mushure memaminitsi matatu mutsa pombi yevacumm.Isa iyo 70kpa.

Mushure memaminetsi matatu, dzima mhepo kune atomizer, asi siya vacuum pombi ichimhanya kwe1 min.

Dzima pombi yevaccum.

Bvisa magungano emuenzaniso kubva mukamuri.Uye neseptically fambisa iyo 0.45um membranes kumabhodhoro epasirese ane 10ml sterile isotonic saline.

Bvisa nekuzunungusa kwe1 min.Uye gadzira serial dilutions ne sterile saline.(10^-1, 10^-2, 10^-3uye ,10^-4)

Gadzira kunze 1ml maaliquots ega ega diluation mune duplicate uchishandisa zvinovaka muviri agar.

Isa mahwendefa usiku hwose pa37 ± 1 ℃ uye ratidza zvawanikwa uchishandisa reshiyo yehuwandu hwehutachiona hwehutachiona kusvika kuhuwandu hwebhakitiriya hwakapfuura kuburikidza nesample yebvunzo.

Ita zvisungo zvina pane imwe neimwe rudzi rwejira kana chimiro chejira.

3. KUCHENGETA

Sezvinei zvese nemidziyo yemagetsi, chikamu ichi chinofanirwa kushandiswa nemazvo uye kuchengetedza uye kuongororwa kunofanirwa kuitwa nguva nenguva.Kuchengetedza kwakadaro kunovimbisa kushanda kwakachengeteka uye kwakanaka kwemidziyo.

Kugadzirisa nguva nenguva kunosanganisira kuongororwa kunoitwa zvakananga nemushandisi webvunzo uye/kana nevashandi vane mvumo.

Kugadziriswa kwemidziyo ibasa remutengi uye kunofanirwa kuitwa sezvakataurwa nechitsauko chino.

Kutadza kuita zvakakurudzirwa zviito zvekugadzirisa kana kugadzirisa kunoitwa nevanhu vasina mvumo kunogona kubvisa waranti.

1. Muchina unofanirwa kutariswa kudzivirira kuvuza kwekubatanidza kusati kwaedzwa;

2. Kufambisa muchina kunorambidzwa kana uchiishandisa;

3. Sarudza inoenderana magetsi uye voltage.Usanyanya kukwirira kudzivirira kupisa mudziyo;

4. Ndapota taura nesu kuitira kuti tibate munguva iyo muchina wacho usina kurongeka;

5. Inofanira kuva nemhepo yakanaka yemhepo kana muchina uchishanda;

6. Kuchenesa muchina mushure mebvunzo nguva dzese;