1. Isishwankathelo

Jonga kula manani alandelayo ngelixa ufunda esi sahluko.

1.1 Intshayelelo engundoqo

1.1.1 Imigangatho

I-ISO/DIS 22611 Isinxibo sokhuseleko kwii-arhente ezosulelayo-Indlela yokuvavanya ukuchasana nokungena ngee-aerosols ezingcolisekileyo zebhayoloji.

1.1.2 Iinkcukacha

lIjenereyitha yeAerosol:    Atomizer

lIgumbi lokuvezwa:  PMMA

lIndibano yesampula :2, intsimbi engenasici

lImpompo yevacum:Ukuya kuthi ga kwi-80kpa

lUbungakanani: 300mm*300mm*300mm

lUkunikezwa Amandla  :220V 50-60Hz

l Ubungakanani bomatshini: 46cm×93cm×49cm (H)

l Ubunzima Net: 35kg

2. UKUSETYENZISWA KWESIXHOBO

2.1 Amalungiselelo

Beka amacandelo amathathu kwikhabhinethi yokhuseleko lwendalo.Khangela indawo nganye yomatshini wokuvavanya kwaye uqinisekise ukuba zonke iindawo zisebenza kakuhle kwaye zidibanisa kakuhle.

Ukusika iisampulu ezisibhozo njenge-25mm ububanzi bezangqa.

Lungiselela inkcubeko yasebusuku ye-Staphylococcus aureus ngokugqithiselwa kwe-aseptic yebhaktiriya kwi-agar yezakhamzimba (egcinwe kwi-4±1℃) ukuya kumhluzi wezondlo kunye nokufukanyelwa kwi-37±1℃ kwi-orbital shaker.

Nciphisa inkcubeko ibe ngumthamo ofanelekileyo wetyuwa ye-isotonic enyumba ukuze unike inani lebhaktiriya lokugqibela elimalunga ne-5*10.⁷iiseli cm^-3usebenzisa igumbi lokubala ibhaktiriya iThoma.

Gcwalisa inkcubeko engentla kwi-atomizer.Inqanaba lolwelo liphakathi kwinqanaba eliphezulu nelisezantsi.

2.2 Ukusebenza

Faka indibano yesampula.Beka i-silicone washer A, ilaphu lokuvavanya, i-silicone washer B, i-membrane, inkxaso yocingo kwisiciko esivulekileyo, gubungela ngesiseko.

Faka enye indibano yesampulu ngaphandle kwesampulu.

Vula isiciko esiphezulu segumbi lovavanyo.

Faka indibano yesampula kunye nesampulu kunye nendibano ngaphandle kwesampulu ngeFasten yeFig. 4-1.

Qinisekisa ukuba zonke iityhubhu ziqhagamshelwe kakuhle.

Qhagamshela umoya oxinanisiweyo kuhlengahlengiso lomoya ocinezelweyo.

Faka isicelo somoya ekuhambeni kwe-5L / min ngokulungelelanisa imitha yokuhamba kwi-atomizer kwaye uqale ukuvelisa i-aerosol.

Emva kwemizuzu emi-3 vula impompo yevacumm.Yimise ibe yi-70kpa.

Emva kwemizuzu emi-3, cima umoya kwi-atomizer, kodwa ushiye i-vacuum pump isebenza kwi-1 min.

Cima impompo yesitofu.

Susa iindibano zesampulu kwigumbi.Kwaye udlulisele nge-aseptically i-0.45um inwebu kwiibhotile zendalo yonke ezine-10ml i-sotonic saline engenazintsholongwane.

Khipha ngokugungqa i-1 min.Kwaye wenze i-serial dilutions nge-saline engenazintsholongwane.(10^-1, 10^-2, 10^-3kunye, kunye ne-10^-4)

Cacisa i-1ml ye-aliquots ye-dilution nganye ngokuphindwe kabini usebenzisa i-agar yezondlo.

Fudumeza iipleyiti ngobusuku obungama-37±1℃ kwaye uveze iziphumo usebenzisa umlinganiselo wokubalwa kwebhaktiriya yangasemva ukuya kwinani lebhaktiriya egqithiselwe kwisampulu yovavanyo.

Yenza izigqibo ezine kuhlobo ngalunye lwelaphu okanye imeko yelaphu.

3. UKULONDOLOZA

Njengazo zonke izixhobo zombane, le yunithi kufuneka isetyenziswe ngokuchanekileyo kwaye ukugcinwa nokuhlolwa kufuneka kwenziwe rhoqo ngamathuba athile.Izilumkiso ezinjalo ziya kuqinisekisa ukusebenza ngokukhuselekileyo nangokufanelekileyo kwesixhobo.

Ukugcinwa kwamaxesha ngamaxesha kubandakanya uhlolo olwenziwe ngokuthe ngqo ngumqhubi wovavanyo kunye/okanye ngabasebenzi benkonzo abagunyazisiweyo.

Ugcino lwezixhobo luxanduva lomthengi kwaye kufuneka lwenziwe njengoko kuchaziwe kwesi sahluko.

Ukungaphumeleli ukwenza izenzo ezicetyiswayo zokulondoloza okanye ukugcinwa okwenziwa ngabantu abangagunyaziswanga kunokuphelisa iwaranti.

1. Umatshini kufuneka utshekishwe ukuthintela ukuvuza koqhagamshelo phambi kovavanyo;

2. Ukuhambisa umatshini akuvumelekanga xa usebenzisa;

3. Khetha umbane ohambelanayo kunye nombane.Musa ukuphakama kakhulu ukunqanda isixhobo esivuthayo;

4. Nceda uqhagamshelane nathi ukuze uphathe ngexesha xa umatshini ungasebenzi;

5. Kufuneka ube nendawo efanelekileyo yokungena komoya xa umatshini usebenza;

6. Ukucoca umatshini emva kovavanyo ngalo lonke ixesha;