1. Incamake

Reba imibare ikurikira mugihe usoma iki gice.

1.1 Intangiriro

1.1.1 Ibipimo

ISO / DIS 22611 Imyenda yo gukingira indwara zanduza-Uburyo bwo gupima uburyo bwo kurwanya kwinjira na aerosole yanduye ibinyabuzima.

1.1.2 Ibisobanuro

lAmashanyarazi:    Atomizer

lIcyumba cyo kumurika:  PMMA

lInteko y'icyitegererezo :2, ibyuma bidafite ingese

lPompe ya Vacumm:Kugera kuri 80kpa

lIgipimo: 300mm * 300mm * 300mm

lAmashanyarazi  :220V 50-60Hz

Igipimo cyimashini: 46cm × 93cm × 49cm （H）

Uburemere bwuzuye: 35kg

2. GUKORESHA IBIKORWA

2.1 Kwitegura

Shira ibice bitatu muri guverenema ya biosafety.Reba buri gice cyimashini yikizamini hanyuma urebe neza ko ibice byose bikora neza kandi bihuza neza.

Gukata ingero umunani nkumuzingi wa 25mm.

Tegura umuco wijoro wa Staphylococcus aureus ukoresheje aseptic yoherejwe na bagiteri iva mu ntungamubiri za agar (zibitswe kuri 4 ± 1 ℃) mu musemburo wintungamubiri hamwe na incubation kuri 37 ± 1 ℃ kuri shitingi ya orbital.

Koresha umuco mubunini bukwiye bwa saline isotonic saline kugirango utange bacteri zanyuma zingana na 5 * 10⁷selile cm^-3ukoresheje icyumba cyo kubara bagiteri ya Thoma.

Uzuza umuco hejuru muri atomizer.Urwego rwamazi ruri hagati yurwego rwo hejuru nu rwego rwo hasi.

2.2 Gukora

Shyiramo inteko y'icyitegererezo.Shira silicone washer A, umwenda wikizamini, silicone washer B, membrane, umugozi winsinga kumupfundikizo ufunguye, upfundikize umusingi.

Shyiramo izindi nteko ntangarugero nta sample.

Fungura umupfundikizo wo hejuru wicyumba cyibizamini.

Shyiramo inteko ntangarugero hamwe nicyitegererezo hamwe ninteko nta sample na Fasten ya shusho 4-1.

Menya neza ko imiyoboro yose ihujwe neza.

Huza umwuka wafunzwe kugirango uhindurwe ikirere.

Koresha umwuka utemba wa 5L / min uhindura metero yatemba kuri atomizer hanyuma utangire kubyara aerosol.

Nyuma yiminota 3 kora pompe ya vacuum.Shyira nka 70kpa.

Nyuma yiminota 3, uzimye umwuka kuri atomizer, ariko usige pompe vacuum ikora muminota 1.

Zimya pompe y'urukingo.

Kuraho inteko ntangarugero mucyumba.Kandi ushishoze wimure 0.45um membrane mumacupa yisi yose arimo 10ml sterile isotonic saline.

Gukuramo ukanyeganyega kuminota 1.Kandi ukore serial serial hamwe na saline sterile.(10^-1, 10^-2, 10^-3, na 10^-4)

Shyira hanze 1ml aliquots ya buri dilution muri duplicate ukoresheje agar intungamubiri.

Shyiramo amasahani ijoro ryose kuri 37 ± 1 ℃ hanyuma ugaragaze ibisubizo ukoresheje igereranyo cyibara rya bacteri yinyuma numubare wa bagiteri yatsinze watsinze ikizamini.

Kora ibyemezo bine kuri buri bwoko bwimyenda cyangwa imiterere.

3. GUKURIKIRA

Kimwe nibikoresho byose byamashanyarazi, iki gice kigomba gukoreshwa neza kandi kubungabunga no kugenzura bigomba gukorwa mugihe gito.Kwirinda nkibi bizemeza imikorere yibikoresho neza kandi neza.

Kubungabunga ibihe bigizwe nubugenzuzi bwakozwe butaziguye nuwashinzwe ikizamini na / cyangwa nabakozi babiherewe uburenganzira.

Kubungabunga ibikoresho ninshingano zumuguzi kandi bigomba gukorwa nkuko byavuzwe niki gice.

Kunanirwa gukora ibikorwa byasabwe byo kubungabunga cyangwa kubungabunga byakozwe nabantu batabifitiye uburenganzira birashobora gukuraho garanti.

1. Imashini igomba kugenzurwa kugirango ikumire guhuza mbere yo gukora ibizamini;

2. Kwimura imashini birabujijwe mugihe uyikoresha;

3. Hitamo amashanyarazi akwiranye na voltage.Ntukabe hejuru cyane kugirango wirinde gutwika ibikoresho;

4. Nyamuneka twandikire kugirango dukemure mugihe imashini idakwiriye;

5. Igomba kugira ibidukikije byiza byo guhumeka mugihe imashini ikora;

6. Kwoza imashini nyuma yikizamini buri gihe;