Hoʻopā i nā ʻāpana mea hana PCR nui Fluorescence

Helu kumu hoʻohālike: CFX96 Touch

1. Ka nohona hana

1.1 Ka wela hana 5-31 ℃

1.2 ʻO ka haʻahaʻa hana ʻO ka haʻahaʻa pili ≤80%

1.3 Ka lako mana hana 100-240 VAC, 50-60Hz.

2. Function

Hiki ke hoʻohana ʻia i ka helu nucleic acid, ka nānā ʻana i ka pae hōʻike gene, ka ʻike mutation gene,

ʻO ka ʻike GMO a me ka nānā ʻana i nā huahana kikoʻī a me nā kahua noiʻi ʻē aʻe.

3. Nā koi hana a me nā ʻenehana

3.1 Hana Nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)

*3.1.1 ʻEono mau ala ʻike, hiki ke hoʻomaopopo i ka PCR 5-fold, hiki ke ʻike i nā ʻano genes 5 i ka manawa like, a me ke kahawai ʻike FRET kūikawā.

*3.1.2 Me ka hana PCR gradient mahana wela, hiki ke holo i 8 mau mahana like ole i ka manawa like, a ua like ka manawa incubation o kela me keia wela.

3.1.3 Reagent hāmama loa, kūpono no nā ʻano noiʻi ʻepekema a me nā reagents lāʻau lapaʻau

3.1.4 He kūpono no nā ʻano fluorescence like ʻole, e like me Taqman, Molecular Beacon, FRET probe, SYBR Green I, etc.

3.1.5 Ua wehe ʻia nā mea hoʻohana a hiki ke hoʻohana i ka paipu hoʻokahi 0.2ml, ʻewalu paipu, pā 96-well, etc.

*3.1.6 Hiki ke holo kūʻokoʻa a hana ma waho.Hiki ke nānā ʻia ka pihi hoʻonui PCR fluorescence manawa maoli me ka ʻole o ka pili kamepiula

3.2 Nā koi ʻenehana nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)

*3.2.1 Laʻana mana: 96×0.2ml, hiki ke hoʻohana maʻamau kikoʻī 96-well pā (12×8)

Ke ʻano o nā mea hoʻopau: 0.2ml paipu hoʻokahi, ʻewalu paipu, 96-well plate, etc

3.2.3 Pūnaehana pane: 1-50µ L (10-25µ L manaʻo ʻia)

*3.2.4 Puna māmā: ʻeono mau alakaʻi me nā kānana

*3.2.5 ʻIke: ʻeono diodes photosensitive me nā kānana

*3.2.6 ka emi ana o ka wela: 5℃/s

3.2.7 Manaʻo hoʻomalu wela: 0-100 ℃

Pono ka wela: ±0.2 ℃ (ma 90˚C)

Kaulike mahana: ± 0.4 ℃ (a hiki i 90˚C i loko o 10 kekona)

*3.2.10 Hana gradient mahana wela: holo 8 mau wela like ʻole i ka manawa like;Ka hoʻomalu ʻana o ka mahana wela: 30-100 ℃;ʻO ke ana wela laulima: 1-24 ℃;Ka manawa incubation ma ka wela gradient: like

3.2.11 Hōʻeuʻeu / hoʻokuʻu ʻia ka lōʻihi hawewe: 450-730nm

3.2.12 ʻIke: Hiki iā ia ke ʻike i nā genes kope hoʻokahi i loko o ka genome kanaka

3.2.13 Ka laulā ikaika: 10 kauoha o ka nui

3.2.14 Hōʻike: 8.5-ʻīniha kala hoʻopā pale

3.2.15 Keʻano hoʻonaʻauaoʻikepili: quantitative maʻamau curve, fusion curve, CT a i ʻole δ δ CT hōʻike hōʻike hōʻikeʻike, ʻike nui i loko o ka hōʻikeʻike gene a me ka hoʻonui ʻana i ka hoʻonui ʻana, ka hōʻike ʻana o nā faila ʻikepili he nui, ka ʻike allele, ka nānā ʻana i ka helu hope, me nā alleles , fusion curve analysis

3.2.16 Hoʻouna ʻikepili: Excel, Word, a i ʻole PowerPoint.Loaʻa i nā hōʻike mea hoʻohana nā ʻōkuhi holo, nā kiʻi a me nā hopena ʻikepili i hiki ke paʻi pololei ʻia a mālama ʻia ma ke ʻano he PDF.

*3.2.17 Haʻawina hoʻolālā Chromosome: Ua hoʻohana ʻia ke ʻano PCR manawa maoli no ka hoʻohālikelike ʻana i ke ʻano o ka chromatin ma o ka hoʻohālikelike ʻana i nā hopena o nā nucleases i ka hōʻino ʻana o DNA genomic.Hōʻike maoli kēia i ka pilina kiʻekiʻe ma waena o ka hoʻolālā chromatin a me ka hōʻike ʻana o ke ʻano

4 Mea Pono

ʻO ka polokalamu kamepiula a me ka nānā ʻana (me ka helu piha, ka helu pili, ka hoʻoheheʻe ʻana i ka curve, ka nānā ʻana i ka hopena, ka hoʻohālikelike ʻikepili multi-board, etc.)

5 Manawa hoʻopaʻa maikaʻi

ʻO ka manawa hōʻoia maikaʻi ʻo hoʻokahi makahiki ma hope o ka hoʻokomo ʻana a me ka ʻae ʻia ʻana o ke kauoha e ka mea hoʻohana.