Touch Fluorescence quantitative PCR lisebelisoa tsa paramente

Nomoro ea mohlala: CFX96 Touch

1. Tikoloho ea mosebetsi

1.1 Mocheso oa ho sebetsa 5-31℃

1.2 Mongobo o sebetsang Mongobo o lekanang ≤80%

1.3 Matla a sebetsang 100-240 VAC, 50-60Hz.

2. Fqholotsoa

E ka sebelisoa ho quantification ea nucleic acid, tlhahlobo ea boemo ba liphatsa tsa lefutso, ho lemoha phetoho ea liphatsa tsa lefutso,

Tlhahlobo ea GMO le tlhahlobo e ikhethang ea sehlahisoa le likarolo tse ling tsa lipatlisiso.

3. Litlhoko tsa ts'ebetso le tekheniki

3.1 Ts'ebetso e ka Sehloohong (* ke letšoao le lokelang ho finyelloa)

* 3.1.1 Mekhoa e tšeletseng ea ho lemoha, e ka khonang ho lemoha 5-fold PCR, ka nako e le 'ngoe e ka lemoha liphatsa tsa lefutso tsa 5, le mocha o khethehileng oa ho lemoha FRET

*3.1.2 Ka ts'ebetso ea PCR ea mocheso o matla, lithempereichara tse 8 tse fapaneng li ka tsamaisoa ka nako e le 'ngoe,' me nako ea ho incubation ea mocheso o mong le o mong oa tšoana.

3.1.3 Reagent e bulehileng ka botlalo, e loketseng mefuta eohle ea lipatlisiso tsa mahlale le lisebelisoa tsa bongaka

3.1.4 E loketse mekhoa e fapaneng ea fluorescence, joalo ka Taqman, Molecular Beacon, FRET probe, SYBR Green I, joalo-joalo.

3.1.5 Lisebelisoa li bulehile 'me li ka sebelisa phala e le' ngoe ea 0.2ml, phala e robeli, poleiti ea seliba sa 96, joalo-joalo.

*3.1.6 E ka sebetsa e ikemetse le ho sebetsa ntle le marang-rang.Nako ea 'nete ea PCR fluorescence amplification curve e ka shejoa ntle le khokahanyo ea khomphutha

3.2 Litlhoko tsa mantlha tsa tekheniki (* ke letšoao le lokelang ho finyelloa)

* 3.2.1 Sample capacity: 96×0.2ml, e ka sebelisoa maemo a tloaelehileng a 96-well plate (12×8)

Mofuta oa lisebelisoa: 0.2ml phala e le 'ngoe, phala e robeli, poleiti ea seliba sa 96, joalo-joalo

3.2.3 Sistimi ea ho arabela: 1-50µ L (10-25µ L e khothalelitsoe)

*3.2.4 Mohloli oa leseli: li-leds tse tšeletseng tse nang le li-filters

* 3.2.5 Detector: li-diode tse tšeletseng tsa lifoto tse nang le li-filters

*3.2.6 mocheso o theoha sekhahla: 5℃/ s

3.2.7 Lebelo la taolo ea mocheso: 0-100 ℃

Ho nepahala ha mocheso: ±0.2℃ (ho 90˚C)

Ho lumellana ha mocheso: ±0.4℃ (ho fihla ho 90˚C nakong ea metsotsoana e 10)

*3.2.10 Ts'ebetso ea matla a matla a mocheso: tsamaisa mocheso o fapaneng oa 8 ka nako e le 'ngoe;Lebelo la taolo ea mocheso oa likhahla: 30-100 ℃;Mocheso oa mocheso oa likhahla: 1-24 ℃;Nako ea ho qhoqhoa ka mocheso oa gradient: ho tšoana

3.2.11 Phatlalatso / wavelength ea mosi: 450-730nm

3.2.12 Sensitivity: E khona ho lemoha kopi e le 'ngoe ea lefutso ho genome ea motho

3.2.13 Dynamic range: 10 orders of magnitude

3.2.14 Pontšo: 8.5-inch color touch screen

3.2.15 Mokhoa oa ho hlahloba lintlha: quantitative standard curve, fusion curve, CT kapa δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification performance calculation, gene expression of multiple data files, allele analysis, end point analysis, with alleles. , fusion curve analysis

3.2.16 Thomello ya Boitsebiso: Excel, Word, kapa PowerPoint.Litlaleho tsa mosebelisi li na le Litlhophiso tse tsamaeang, liphetho tsa data tse hlakileng le tse thathamisitsoeng tse ka hatisoang ka kotloloho kapa tsa bolokoa joalo ka PDF.

*3.2.17 Thuto ea sebopeho sa Chromosome: Mokhoa oa PCR oa nako ea sebele o ile oa sebelisoa ho hlahloba ka bongata sebopeho sa chromatin ka ho bapisa liphello tsa li-nuclease ho senyeha ha DNA ea genomic.Sena se hlile se bonts'a kamano e phahameng lipakeng tsa sebopeho sa chromatin le polelo ea lefutso

4 Lisebelisoa tse Hlokahalang

Software ea tlhahlobo ea khomphutha le taolo (ho kenyeletsoa palo e felletseng, palo e lekanang, tlhahlobo ea curve e qhibilihang, tlhahlobo ea lintlha, papiso ea lintlha tse ngata, jj.)

5 Quality Guarantee nako

Nako ea tiiso ea boleng e tla ba selemo se le seng ka mor'a hore ho kenngoa le ho romelloa ho amoheloe le ho amoheloa ke mosebedisi.