Thinta amapharamitha wethuluzi le-PCR lomthamo we-Fluorescence

Inombolo yemodeli: CFX96 Touch

1. Indawo yokusebenza

1.1 Izinga lokushisa lokusebenza 5-31℃

1.2 Umswakamo Osebenzayo Umswakama ohlobene ≤80%

1.3 Amandla asebenzayo 100-240 VAC, 50-60Hz.

2. Fukuqaqa

Ingasetshenziswa ku-nucleic acid quantification, ukuhlaziywa kwezinga lesisho sofuzo, ukutholwa kokuguqulwa kofuzo,

Ukutholwa kwe-GMO nokuhlaziywa kwemininingwane yomkhiqizo kanye neminye imikhakha yocwaningo.

3. Izidingo zokusebenza kanye nezobuchwepheshe

3.1 Ukusebenza Okubalulekile (* inkomba okufanele kuhlangatshezwane nayo)

*3.1.1 Iziteshi zokuthola eziyisithupha, ezikwazi ukubona i-PCR ephindwe ka-5, zingathola ngasikhathi sinye izakhi zofuzo eziqondiwe ezi-5, kanye nesiteshi esikhethekile sokuthola i-FRET

*3.1.2 Ngokusebenza kwe-PCR yezinga lokushisa eliguquguqukayo, amazinga okushisa angu-8 ahlukene angaqhutshwa ngesikhathi esisodwa, futhi isikhathi sokufukamela sezinga lokushisa ngalinye siyefana

3.1.3 I-reagent evuleke ngokuphelele, elungele zonke izinhlobo zocwaningo lwesayensi kanye nezinto ezisebenza ngomtholampilo

3.1.4 Ifanele izindlela ezehlukene ze-fluorescence, njenge-Taqman, i-Molecular Beacon, i-FRET probe, i-SYBR Green I, njll.

3.1.5 Izinto ezisetshenziswayo zivuliwe futhi zingasebenzisa ipayipi elilodwa elingu-0.2ml, ipayipi eliyisishiyagalombili, ipuleti lemithombo engu-96, njll.

*3.1.6 Ingasebenza ngokuzimela futhi isebenze ingaxhunyiwe ku-inthanethi.Ijika le-PCR fluorescence amplification yesikhathi sangempela ingagadwa ngaphandle koxhumano lwekhompyutha

3.2 Izidingo zobuchwepheshe eziyinhloko (* inkomba okufanele ihlangatshezwe)

*3.2.1 Umthamo wesampula: 96×0.2ml, ungasetshenziswa ukucaciswa okujwayelekile okungu-96-well plate (12×8)

Uhlobo lwezinto ezisetshenziswayo: 0.2ml ipayipi elilodwa, ipayipi eliyisishiyagalombili, ipuleti lemithombo engama-96, njll

3.2.3 Isistimu yokusabela: 1-50µ L (10-25µ L iyanconywa)

*3.2.4 Umthombo wokukhanya: amaledi ayisithupha anezihlungi

*3.2.5 Umtshina: ama-diode ayisithupha e-photosensitive anezihlungi

*3.2.6 izinga lokushisa lokwehla: 5℃/ s

3.2.7 Ibanga lokulawula izinga lokushisa: 0-100 ℃

Ukunemba kwezinga lokushisa: ±0.2℃ (ku-90˚C)

Ukufana kwezinga lokushisa: ±0.4℃ (kufika ku-90˚C phakathi kwamasekhondi angu-10)

*3.2.10 Umsebenzi we-gradient wokushisa oguqukayo: sebenzisa amazinga okushisa angu-8 ahlukene ngesikhathi esisodwa;Ibanga lokulawula izinga lokushisa le-gradient: 30-100 ℃;Ibanga lokushisa le-gradient: 1-24 ℃;Isikhathi sokufukamela ezingeni lokushisa le-gradient: okufanayo

3.2.11 Ububanzi be-wavelength ye-excitation/ekhiphayo: 450-730nm

3.2.12 Ukuzwela: Ingakwazi ukubona ikhophi eyodwa yofuzo ku-genome yomuntu

3.2.13 Ibanga elinamandla: ama-oda ayi-10 obukhulu

3.2.14 Isibonisi: 8.5-intshi umbala touch screen

3.2.15 Imodi yokuhlaziya idatha: ijika elijwayelekile lobuningi, ijika elihlanganisiwe, ukuhlaziywa kwe-CT noma δ δ CT CT, ukuhlaziywa kwezakhi zofuzo eziningi zereferensi nokubalwa kokusebenza kahle kokukhulisa, ukuhlaziya isisho sofuzo samafayela edatha amaningi, ukuhlaziywa kwe-allele, ukuhlaziya iphuzu lokugcina, nama-alleles. , ukuhlaziya ijika le-fusion

3.2.16 Ukuthunyelwa Kwedatha: Excel, Word, noma PowerPoint.Imibiko yomsebenzisi iqukethe qalisa Izilungiselelo, imiphumela yedatha enemifanekiso nethebula engaphrintwa ngokuqondile noma ilondolozwe njenge-PDF.

*3.2.17 Ucwaningo lwesakhiwo se-Chromosome: Indlela ye-PCR yesikhathi sangempela yasetshenziswa ukuze kuhlaziywe ngokwesilinganiso ukwakheka kwe-chromatin ngokuqhathanisa imiphumela yama-nuclease ekonakaleni kwe-genomic DNA.Lokhu kukhombisa ngempela ukuhlobana okuphezulu phakathi kwesakhiwo se-chromatin nesisho sofuzo

4 Izesekeli Ezidingekayo

Isofthiwe yokuhlaziya ikhompuyutha nokulawula (okuhlanganisa ukulinganisa okuphelele, ukulinganisa okuhlobene, ukuhlaziya ijika elincibilikayo, ukuhlaziya amaphuzu wokugcina, ukuqhathanisa idatha yamabhodi amaningi, njll.)

5 Isikhathi sokuqinisekisa ikhwalithi

Isikhathi sesiqinisekiso sekhwalithi sizoba unyaka owodwa ngemuva kokuthi ukufakwa nokusetshenziswa kwamukelwe futhi kwamukelwe umsebenzisi.