Gukoraho Fluorescence igizwe nibikoresho bya PCR
Ibisobanuro bigufi:
Umubare w'icyitegererezo: CFX96 Gukoraho 1. Ibidukikije by'akazi 1.1 Ubushyuhe bukora 5-31 ℃ 1.2 Ubushuhe bukoresha Ubushuhe bugereranije ≤80% 1.3 Amashanyarazi akora 100-240 VAC, 50-60Hz.2. Imikorere Irashobora gukoreshwa mukugereranya acide nucleic, gusesengura urwego rwa gene, gusesengura gene mutation, kumenya GMO no gusesengura ibicuruzwa byihariye nubundi bushakashatsi.3. Imikorere nibisabwa tekinike 3.1 Imikorere nyamukuru (* nicyo kimenyetso kigomba kuba cyujuje) * 3.1.1 Channe itandatu yo kumenya ...
Inomero yicyitegererezo: CFX96 Gukoraho
1. Ibidukikije
1.1 Ubushyuhe bukora 5-31 ℃
1.2 Gukoresha Ubushuhe Ubushuhe bugereranije ≤80%
1.3 Amashanyarazi akora 100-240 VAC, 50-60Hz.
2. Function
Irashobora gukoreshwa mukugereranya aside nucleic, gusesengura urwego rwa gene, gusesengura gene mutation,
Gushakisha GMO no gusesengura ibicuruzwa byihariye nubundi bushakashatsi.
3. Imikorere n'ibisabwa tekinike
3.1 Imikorere nyamukuru (* nicyo kimenyetso kigomba kubahirizwa)
* 3.1.1 Imiyoboro itandatu yo gutahura, ishobora kumenya PCR inshuro 5, irashobora icyarimwe kumenya genes 5 zigenewe, hamwe numuyoboro wihariye wa FRET
* 3.1.
3.1.3 Fungura reagent yuzuye, ibereye ubwoko bwubushakashatsi bwa siyanse na reagent
3.1.4 Birakwiriye muburyo butandukanye bwa fluorescence, nka Taqman, Beacon ya Molecular, FRET probe, SYBR Green I, nibindi
3.1.5 Ibikoreshwa birakinguye kandi birashobora gukoresha 0.2ml umuyoboro umwe, umuyoboro umunani, isahani 96-nziza, nibindi
* 3.1.6 Irashobora gukora yigenga kandi ikora kumurongo.Igihe nyacyo PCR fluorescence amplification curve irashobora gukurikiranwa nta mudasobwa ihuza
3.2 Ibisabwa byingenzi bya tekiniki (* nicyo kimenyetso kigomba kuba cyujuje)
* 3.2.1 Ubushobozi bw'icyitegererezo: 96 × 0.2ml, burashobora gukoreshwa ibisobanuro bisanzwe 96 isahani (12 × 8)
Ubwoko bwibikoreshwa: 0.2ml umuyoboro umwe, umuyoboro umunani, isahani 96-nziza, nibindi
3.2.3 Sisitemu yo kubyitwaramo: 1-50µ L (10-25µ L isabwa)
* 3.2.4 Inkomoko yumucyo: ibitanda bitandatu hamwe nayunguruzo
* 3.2.5 Detector: diode esheshatu zifotora hamwe na filteri
* 3.2.6 igipimo cyo kugabanuka k'ubushyuhe: 5 ℃ / s
3.2.7 Urwego rwo kugenzura ubushyuhe: 0-100 ℃
Ubushyuhe bwuzuye: ± 0.2 ℃ (kuri 90˚C)
Uburinganire bwubushyuhe: ± 0.4 ℃ (kugeza 90˚C mumasegonda 10)
* 3.2.10 Imikorere yubushyuhe buringaniye: koresha ubushyuhe 8 butandukanye icyarimwe;Kugenzura ubushyuhe bwa Gradient: 30-100 ℃;Ubushyuhe buringaniye: 1-24 ℃;Igihe cyo gukuramo ubushyuhe bwa gradient: kimwe
3.2.11 Ibyishimo / imyuka yoherejwe: 450-730nm
3.2.12 Ibyiyumvo: Irashobora kumenya ingirabuzimafatizo imwe muri genomuntu
3.2.13 Urwego rudasanzwe: amategeko 10 yubunini
3.2.14 Erekana: ecran ya 8.5-yimyenda ikoraho
3.2.15. , gusesengura umurongo
3.2.16 Kohereza amakuru hanze: Excel, Ijambo, cyangwa PowerPoint.Raporo y'abakoresha ikubiyemo gukora Igenamiterere, ibishushanyo mbonera hamwe n'ibisubizo byatanzwe bishobora gucapurwa mu buryo butaziguye cyangwa bikabikwa nka PDF
* 3.2.17 Ubushakashatsi bwa Chromosome: Uburyo nyabwo bwa PCR bwakoreshejwe mu gusesengura mu buryo bwuzuye imiterere ya chromatine ugereranya ingaruka za nuclease ku iyangirika rya ADN.Ibi rwose birerekana isano iri hagati yimiterere ya chromatin nimvugo ya gene
4 Ibikoresho bya ngombwa
Porogaramu yo gusesengura mudasobwa no kugenzura (harimo kugereranya byuzuye, kugereranya ugereranije, gushonga umurongo wo gusesengura, gusesengura amaherezo, kugereranya amakuru menshi, kugereranya, n'ibindi)
Igihe cyubwishingizi bwiza
Igihe cyubwishingizi bufite ireme kizaba umwaka umwe nyuma yo kwishyiriraho no gutangiza byemewe kandi byemerwa numukoresha.
