Kukhudza kwa Fluorescence kuchuluka kwa zida za PCR

Nambala yachitsanzo: CFX96 Touch

1. Malo ogwirira ntchito

1.1 Kutentha kwa ntchito 5-31 ℃

1.2 Chinyezi Chogwira Ntchito Chinyezi Chachibale ≤80%

1.3 Mphamvu yogwira ntchito 100-240 VAC, 50-60Hz.

2. Fkukomoka

Itha kugwiritsidwa ntchito mu nucleic acid quantification, gene expression level, gene mutation kuzindikira,

Kuzindikira kwa GMO ndi kusanthula kwatsatanetsatane wazinthu ndi magawo ena ofufuza.

3. Zofunikira zantchito ndi luso

3.1 Main Performance (* ndiye chizindikiro chomwe chiyenera kukumana)

* 3.1.1 Njira zisanu ndi imodzi zodziwira, zomwe zimatha kuzindikira 5-fold PCR, zimatha kuzindikira nthawi imodzi jini 5, ndi njira yapadera yodziwira FRET

* 3.1.2 Ndi ntchito ya PCR yosinthasintha kutentha, kutentha kwa 8 kumatha kuyendetsedwa nthawi imodzi, ndipo nthawi yoyikira kutentha kulikonse ndi yofanana.

3.1.3 Reagent yotseguka kwathunthu, yoyenera mitundu yonse ya kafukufuku wasayansi ndi ma reagents azachipatala

3.1.4 Yoyenera njira zosiyanasiyana za fluorescence, monga Taqman, Molecular Beacon, FRET probe, SYBR Green I, ndi zina zotero.

3.1.5 Consumables ndi lotseguka ndipo angagwiritse ntchito 0.2ml limodzi chitoliro, eyiti chitoliro, 96-chitsime mbale, etc.

* 3.1.6 Imatha kuyendetsa palokha ndikugwira ntchito popanda intaneti.Real-time PCR fluorescence amplification curve imatha kuyang'aniridwa popanda kulumikizana ndi kompyuta

3.2 Zofunikira zazikulu zaukadaulo (* ndiye chizindikiro chomwe chiyenera kukwaniritsidwa)

* 3.2.1 chitsanzo mphamvu: 96 × 0.2ml, angagwiritsidwe ntchito muyezo mfundo 96-chitsime mbale (12 × 8)

Mtundu wa consumables: 0.2ml chitoliro chimodzi, chitoliro eyiti, 96-chitsime mbale, etc

3.2.3 Dongosolo lochitira: 1-50µ L (10-25µ L yovomerezeka)

* 3.2.4 Gwero la kuwala: ma LED asanu ndi limodzi okhala ndi zosefera

*3.2.5 Detector: ma diode asanu ndi limodzi okhala ndi zosefera

* Kutsika kwa kutentha kwa 3.2.6: 5 ℃/ s

3.2.7 Kutentha kosiyanasiyana: 0-100 ℃

Kutentha kolondola: ±0.2℃ (pa 90˚C)

Kutentha kofanana: ± 0.4 ℃ (mpaka 90˚C mkati mwa masekondi 10)

* 3.2.10 Kutentha kwamphamvu kwa gradient ntchito: kuthamanga 8 kutentha kosiyana nthawi imodzi;gradient kutentha kulamulira osiyanasiyana: 30-100 ℃;gradient kutentha osiyanasiyana: 1-24 ℃;Makulitsidwe nthawi pa gradient kutentha: chimodzimodzi

3.2.11 Chisangalalo / utsi wavelength osiyanasiyana: 450-730nm

3.2.12 Sensitivity: Imatha kuzindikira jini limodzi mu genome ya munthu

3.2.13 Dynamic range: 10 orders of magnitude

3.2.14 Sonyezani: 8.5-inchi mtundu kukhudza chophimba

3.2.15 Njira yowunikira deta: kuchuluka kwa curve, fusion curve, CT kapena δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification performance calculation, gene expression of multiple data files, allele analysis, endpoint analysis, ndi alleles. , fusion curve kusanthula

3.2.16 Kutumiza Kwa data: Excel, Mawu, kapena PowerPoint.Malipoti a ogwiritsa ntchito ali ndi Zikhazikiko zoyendetsa, zotsatira zazithunzi ndi zolemba zomwe zitha kusindikizidwa mwachindunji kapena kusungidwa ngati PDF.

*3.2.17 Kapangidwe ka chromosome: Njira ya PCR ya nthawi yeniyeni idagwiritsidwa ntchito kusanthula kachulukidwe ka chromatin poyerekeza zotsatira za ma nuclease pakuwonongeka kwa genomic DNA.Izi zikuwonetsa kulumikizana kwakukulu pakati pa kapangidwe ka chromatin ndi mawonekedwe a jini

4 Zofunikira Zofunikira

Mapulogalamu osanthula makompyuta ndi owongolera (kuphatikiza kuchuluka kwathunthu, kuchuluka kwachibale, kusanthula kwa ma curve, kusanthula kwa ma endpoint, kufananitsa deta yama board ambiri, ndi zina zambiri.)

5 Quality Guarantee nthawi

Nthawi yotsimikizira zaubwino idzakhala chaka chimodzi kukhazikitsidwa ndi kutumiza kuvomerezedwa ndikuvomerezedwa ndi wogwiritsa ntchito.