Kov Fluorescence quantitative PCR ntsuas ntsuas

Qauv nab npawb: CFX96 Kov

1. Qhov chaw ua haujlwm

1.1 Ua haujlwm kub 5-31 ℃

1.2 Kev khiav hauj lwm Humidity txheeb ze humidity ≤80%

1.3 Ua hauj lwm fais fab mov 100-240 VAC, 50-60Hz.

2. Ftsis ua haujlwm

Nws tuaj yeem siv rau hauv nucleic acid kom muaj nuj nqis, gene qhia theem tsom xam, gene mutation detection,

Kev tshawb nrhiav GMO thiab kev txheeb xyuas cov khoom tshwj xeeb thiab lwm yam kev tshawb fawb.

3. Kev ua tau zoo thiab kev xav tau

3.1 Main Performance (* yog qhov taw qhia uas yuav tsum tau ua)

* 3.1.1 Rau qhov kev tshawb nrhiav, uas tuaj yeem paub txog 5-fold PCR, tuaj yeem tshawb pom 5 lub hom phiaj tib lub sijhawm, thiab tshwj xeeb FRET nrhiav kom pom channel

* 3.1.2 Nrog rau qhov kub thiab txias gradient PCR muaj nuj nqi, 8 qhov kub sib txawv tuaj yeem khiav tib lub sijhawm, thiab lub sijhawm incubation ntawm txhua qhov kub yog tib yam.

3.1.3 Ua tiav qhib reagent, haum rau txhua yam kev tshawb fawb thiab kev kho mob reagents

3.1.4 Haum rau ntau txoj kev fluorescence, xws li Taqman, Molecular Beacon, FRET sojntsuam, SYBR Green I, thiab lwm yam

3.1.5 Consumables qhib thiab siv tau 0.2ml ib leeg yeeb nkab, yim yeeb nkab, 96-zoo phaj, thiab lwm yam

* 3.1.6 tuaj yeem khiav ntawm nws tus kheej thiab ua haujlwm offline.Real-time PCR fluorescence amplification nkhaus tuaj yeem saib xyuas yam tsis muaj kev sib txuas hauv computer

3.2 Cov kev cai tseem ceeb (* yog qhov taw qhia uas yuav tsum tau ua)

* 3.2.1 Qauv muaj peev xwm: 96 × 0.2ml, tuaj yeem siv tus qauv specification 96-zoo phaj (12 × 8)

Hom khoom siv: 0.2ml ib leeg yeeb nkab, yim yeeb nkab, 96-zoo phaj, thiab lwm yam

3.2.3 Reaction system: 1-50µL (10-25µL pom zoo)

* 3.2.4 Lub teeb qhov chaw: rau leds nrog lim

* 3.2.5 Ntes: 6 photosensitive diodes nrog cov ntxaij lim dej

* 3.2.6 kub poob tus nqi: 5 ℃ / s

3.2.7 Kev tswj qhov kub thiab txias: 0-100 ℃

Kub qhov tseeb: ± 0.2 ℃ (ntawm 90˚C)

Kev ntsuas kub: ± 0.4 ℃ (txog 90˚C hauv 10 vib nas this)

* 3.2.10 Dynamic kub gradient muaj nuj nqi: khiav 8 qhov kub sib txawv tib lub sijhawm;Gradient kub tswj ntau yam: 30-100 ℃;Gradient kub ntau yam: 1-24 ℃;Lub sij hawm incubation ntawm gradient kub: tib yam

3.2.11 Excitation / emission wavelength ntau: 450-730nm

3.2.12 rhiab heev: Nws tuaj yeem kuaj pom tib cov noob caj noob ces hauv tib neeg genome

3.2.13 Dynamic ntau yam: 10 xaj ntawm qhov loj

3.2.14 Zaub: 8.5-nti xim kov npo

3.2.15 Cov ntaub ntawv tsom xam hom: kom muaj nuj nqis tus qauv nkhaus, fusion nkhaus, CT lossis δ δ CT gene qhia kev tsom xam, ntau yam kev siv noob caj noob ces thiab kev ua kom muaj txiaj ntsig zoo suav nrog, gene qhia kev txheeb xyuas ntawm ntau cov ntaub ntawv, allele tsom, qhov kawg taw tes tsom, nrog alleles , fusion nkhaus tsom xam

3.2.16 Cov Ntaub Ntawv Export: Excel, Lo Lus, lossis PowerPoint.Cov ntawv ceeb toom cov neeg siv muaj cov chaw ua haujlwm, cov duab kos duab thiab cov ntaub ntawv tabulated uas tuaj yeem luam tawm ncaj qha lossis khaws cia ua PDF

* 3.2.17 Chromosome qauv kawm: Real-time PCR txoj kev tau siv los txheeb xyuas cov qauv chromatin los ntawm kev sib piv cov teebmeem ntawm nucleases ntawm genomic DNA degradation.Qhov no qhia tau hais tias muaj kev sib raug zoo ntawm cov qauv chromatin thiab gene qhia

4 Yam khoom tsim nyog

Khoos phis tawj thiab kev tswj xyuas software (nrog rau cov khoom kom muaj nuj nqis, txheeb xyuas qhov sib npaug, kev txheeb xyuas qhov nkhaus, kev txheeb xyuas qhov kawg, kev sib piv cov ntaub ntawv ntau, thiab lwm yam)

5 Lub sijhawm lav zoo

Lub sij hawm lav zoo yuav yog ib xyoos tom qab lub installation thiab commissioning tau txais thiab txais los ntawm cov neeg siv.