Touch Fluorescence quantitative PCR instrument parameters

Model number: CFX96 Touch

1. The work environment

1.1 Operating temperature 5-31℃

1.2 Operating Humidity Relative humidity ≤80%

1.3 Working power supply 100-240 VAC, 50-60Hz.

2. Function

It can be used in nucleic acid quantification, gene expression level analysis, gene mutation detection,

GMO detection and product specificity analysis and other research fields.

3. Performance and technical requirements

3.1 Main Performance (* is the indicator that must be met)

*3.1.1 Six detection channels, which can realize 5-fold PCR, can simultaneously detect 5 target genes, and special FRET detection channel

*3.1.2 With dynamic temperature gradient PCR function, 8 different temperatures can be run at the same time, and the incubation time of each temperature is the same

3.1.3 Completely open reagent, suitable for all kinds of scientific research and clinical reagents

3.1.4 Suitable for a variety of fluorescence methods, such as Taqman, Molecular Beacon, FRET probe, SYBR Green I, etc

3.1.5 Consumables are open and can use 0.2ml single pipe, eight pipe, 96-well plate, etc

*3.1.6 Can run independently and operate offline. Real-time PCR fluorescence amplification curve can be monitored without computer connection

3.2 Main technical requirements (* is the indicator that must be met)

*3.2.1 Sample capacity: 96×0.2ml, can be used standard specification 96-well plate (12×8)

Type of consumables: 0.2ml single pipe, eight pipe, 96-well plate, etc

3.2.3 Reaction system: 1-50µ L (10-25µ L recommended)

*3.2.4 Light source: six leds with filters

*3.2.5 Detector: six photosensitive diodes with filters

*3.2.6 temperature drop rate: 5℃/ s

3.2.7 Temperature control range: 0-100 ℃

Temperature accuracy: ±0.2℃ (at 90˚C)

Temperature uniformity: ±0.4℃ (up to 90˚C within 10 seconds)

*3.2.10 Dynamic temperature gradient function: run 8 different temperatures at the same time; Gradient temperature control range: 30-100 ℃; Gradient temperature range: 1-24 ℃; Incubation time at gradient temperature: same

3.2.11 Excitation/emission wavelength range: 450-730nm

3.2.12 Sensitivity: It can detect single copy genes in human genome

3.2.13 Dynamic range: 10 orders of magnitude

3.2.14 Display: 8.5-inch color touch screen

3.2.15 Data analysis mode: quantitative standard curve, fusion curve, CT or δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification efficiency calculation, gene expression analysis of multiple data files, allele analysis, end point analysis, with alleles, fusion curve analysis

3.2.16 Data Export: Excel, Word, or PowerPoint. User reports contain run Settings, graphical and tabulated data results that can be printed directly or saved as a PDF

*3.2.17 Chromosome structure study: Real-time PCR method was used to quantitatively analyze chromatin structure by comparing the effects of nucleases on genomic DNA degradation. This really demonstrates a high correlation between chromatin structure and gene expression

4 Necessary Accessories

Computer and control analysis software (including absolute quantification, relative quantification, melting curve analysis, endpoint analysis, multi-board data comparison, etc.)

5 Quality Guarantee period

The quality guarantee period will be one year after the installation and commissioning is accepted and accepted by the user.