Pa'i Fluorescence quantitative PCR mea faigaluega tapula'a

Numera fa'atusa: CFX96 Touch

1. Le siosiomaga o galuega

1.1 Gaioiina vevela 5-31 ℃

1.2 Susū Fa'agaoioi Susū fa'atusatusa ≤80%

1.3 Galulue eletise 100-240 VAC, 50-60Hz.

2. Function

E mafai ona faʻaaogaina i le faʻatusatusaina o le nucleic acid, suʻesuʻega o faʻamatalaga o le faʻaaliga, suʻesuʻeina o suiga o le gene,

Su'esu'ega GMO ma au'ili'iliga fa'apitoa o oloa ma isi matā'upu su'esu'e.

3. Fa'atinoga ma mana'oga fa'apitoa

3.1 Fa'atinoga Autu (* o le fa'ailoga e tatau ona fa'amalieina)

*3.1.1 E ono auala e iloa ai, lea e mafai ona iloa ai le PCR 5-fold, e mafai i le taimi e tasi ona iloa 5 kenera taulaʻi, ma faʻapitoa FRET iloa auala.

*3.1.2 Faatasi ai ma le malosi o le vevela gradient PCR galuega, 8 eseese vevela e mafai ona tamoe i le taimi e tasi, ma le taimi incubation o vevela taitasi e tutusa

3.1.3 Reagent tatala atoatoa, talafeagai mo ituaiga uma o suesuega faasaienisi ma reagents falemai

3.1.4 E talafeagai mo auala eseese o le fluorescence, e pei ole Taqman, Molecular Beacon, FRET probe, SYBR Green I, ma isi.

3.1.5 O lo'o avanoa mea fa'aaoga ma e mafai ona fa'aoga 0.2ml paipa ta'itasi, valu paipa, 96-well plate, etc.

*3.1.6 E mafai ona tamo'e tuto'atasi ma fa'agaioi tuusao.Ole taimi moni ole PCR fluorescence amplification curve e mafai ona mata'ituina e aunoa ma se feso'ota'iga komepiuta

3.2 Manaoga faʻapitoa faʻapitoa (* o le faʻailoga e tatau ona ausia)

* 3.2.1 Faʻataʻitaʻiga gafatia: 96 × 0.2ml, e mafai ona faʻaaogaina faʻamatalaga masani 96-well plate (12 × 8)

Ituaiga o consumables: 0.2ml paipa tasi, valu paipa, 96-well ipu, ma isi

3.2.3 Reaction system: 1-50µ L (10-25µ L fautuaina)

*3.2.4 Punavai malamalama: ono ta'ita'i ma faamama

*3.2.5 Su'esu'e: ono diodes photosensitive ma faamama

*3.2.6 fua faatatau pa'u o le vevela: 5℃/s

3.2.7 Fa'atonuga o le vevela: 0-100 ℃

Sa'o o le vevela: ±0.2℃ (i le 90˚C)

Tulaga tutusa o le vevela: ± 0.4 ℃ (e oo i le 90˚C i totonu ole 10 sekone)

*3.2.10 Fa'agaioiga fa'agasologa o le vevela: fa'atautaia 8 vevela eseese i le taimi e tasi;Fa'asa'o fa'atonuga o le vevela: 30-100 ℃;Fa'alili le vevela va'aiga: 1-24 ℃;Taimi fa'a'a'ai ile vevela fa'alili: tutusa

3.2.11 Fa'afiafiaga/fa'asa'olotoga umi galu: 450-730nm

3.2.12 Ma'ale'ale: E mafai ona iloa kopi kopi tasi kenera i genome tagata

3.2.13 Va'aiga malosi: 10 poloaiga o le tele

3.2.14 Fa'aaliga: 8.5-inisi lanu pa'i mata

3.2.15 Faiga su'esu'e o fa'amaumauga: fa'avasegaina fa'afuainumera fa'atatau, fusuaga fusu'aga, CT po'o le δ δ CT su'esu'ega fa'amatalaga fa'atupuina, tele fa'asinomaga fa'alotoifale su'esu'ega ma le fa'atuputeleina lelei fa'atatau, su'esu'ega fa'amatalaga o le tele o faila fa'amaumauga, su'esu'ega allele, su'esu'ega pito i'uga, fa'atasi ai ma alleles , su'esu'ega pi'o fusi

3.2.16 Fa'asalalau Fa'amatalaga: Excel, Word, po'o le PowerPoint.O lipoti a le tagata fa'aoga o lo'o i ai Fa'atonuga ta'avale, fa'asologa fa'asologa ma fa'amaumauga fa'amaumauga e mafai ona lolomi sa'o pe fa'asaoina o se PDF.

*3.2.17 Su'esu'ega o fausaga o Chromosome: Na fa'aogaina le auala moni ole PCR e su'esu'e fa'atatau le fausaga o le chromatin e ala i le fa'atusatusaina o a'afiaga o nucleases ile genomic DNA degradation.O lenei mea e faʻaalia moni ai le maualuga o le fesoʻotaʻiga i le va o le chromatin structure ma le faʻaaliga o gene

4 Mea Fa'aopoopo Manaomia

Komipiuta ma fa'atonu polokalame su'esu'ega (e aofia ai le fa'atusatusaina atoatoa, fa'atusatusaga fa'atatau, su'esu'ega fa'afefeteina, su'esu'ega pito, fa'atusatusaga o fa'amaumauga e tele-board, ma isi)

5 Vaitaimi Fa'amautinoaga Lelei

Ole taimi ole fa'amautinoaga lelei ole a tasi le tausaga talu ona fa'apipi'iina ma fa'atonuina e talia ma talia e le tagata fa'aoga.