Metụ Fluorescence quantitative PCR ngwá ọrụ

Nọmba nlereanya: CFX96 Touch

1. Ebe ọrụ

1.1 Ọnọdụ okpomọkụ 5-31 ℃

1.2 iru iru mmiri na-arụ ọrụ ≤80%

1.3 Ike ọkụ na-arụ ọrụ 100-240 VAC, 50-60Hz.

2. Fnjikọ

Enwere ike iji ya na ọnụọgụ nucleic acid, nyocha ọkwa mkpụrụ ndụ ihe nketa, nchọpụta mkpụrụ ndụ ihe nketa,

Nchọpụta GMO na nyocha nkọwapụta ngwaahịa na mpaghara nyocha ndị ọzọ.

3. Arụmọrụ na teknụzụ chọrọ

3.1 Isi arụmọrụ (* bụ egosi nke a ga-ezute)

* 3.1.1 Ọwa nchọpụta isii, nke nwere ike ịghọta PCR ugboro ise, nwere ike ịchọpụta mkpụrụ ndụ ihe nketa 5 n'otu oge, yana ọwa nchọpụta FRET pụrụ iche.

* 3.1.2 Site na ọrụ PCR gradient okpomọkụ dị ike, enwere ike ịgba ọsọ 8 dị iche iche n'otu oge, na oge ntinye nke okpomọkụ ọ bụla bụ otu.

3.1.3 meghere reagent zuru oke, dabara maka ụdị nyocha sayensị niile na reagents ụlọ ọgwụ.

3.1.4 Kwesịrị ekwesị maka ụzọ dị iche iche nke fluorescence, dị ka Taqman, Molecular Beacon, FRET probe, SYBR Green I, wdg.

3.1.5 Consumables na-emeghe na ike iji 0.2ml otu ọkpọkọ, asatọ anwụrụ, 96-ọma efere, wdg

* 3.1.6 nwere ike na-agba ọsọ n'onwe ya ma rụọ ọrụ na-anọghị n'ịntanetị.Enwere ike nyochaa usoro mmụba fluorescence PCR n'ezie na-enweghị njikọ kọmputa

3.2 Isi teknụzụ chọrọ (* bụ ihe ngosi a ga-emezurịrị)

* 3.2.1 Sample ike: 96 × 0.2ml, ike ga-eji ọkọlọtọ nkọwapụta 96-ọma efere (12×8)

Ụdị consumables: 0.2ml otu ọkpọkọ, asatọ ọkpọkọ, 96-ọma efere, wdg

3.2.3 Sistemụ mmeghachi omume: 1-50µ L (10-25µ L akwadoro)

* 3.2.4 Isi iyi ọkụ: ụzọ isii nwere nzacha

* 3.2.5 Onye nchọpụta: diode foto mmetụta isii nwere nzacha

* 3.2.6 okpomọkụ ọdịda ọnụego: 5 ℃ / s

3.2.7 Oke njikwa okpomọkụ: 0-100 ℃

Okpomọkụ ziri ezi: ± 0.2℃ (na 90˚C)

Ịdị n'otu okpomọkụ: ± 0.4℃ (ruo 90˚C n'ime 10 sekọnd)

* 3.2.10 Dynamic okpomọkụ gradient ọrụ: na-agba ọsọ 8 dị iche iche okpomọkụ n'otu oge;Oke njikwa okpomọkụ gradient: 30-100 ℃;Ọnọdụ okpomọkụ gradient: 1-24 ℃;Oge nnabata na gradient okpomọkụ: otu

3.2.11 Ogologo oge mkpali/mịpụta: 450-730nm

3.2.12 Mmetụta: Ọ nwere ike ịchọpụta otu mkpụrụ ndụ ihe nketa na genome mmadụ

3.2.13 Oke dị ike: 10 iwu nke ịdị ukwuu

3.2.14 Ngosipụta: 8.5-inch agba ihuenyo mmetụ

3.2.15 Data analysis mode: quantitative standard curve, fusion curve, CT or δ δ CT gene expression analysis, multiple internal reference gene analysis and amplification rating rating, gene expression analysis of multiple data files, allele analysis, end point analysis, with alleles. , fusion curve analysis

3.2.16 Mbupụ data: Excel, Okwu, ma ọ bụ PowerPoint.Akụkọ onye ọrụ nwere ntọala ọsọ, eserese na nsonaazụ data enwere ike ibipụta ozugbo ma ọ bụ chekwaa dị ka PDF.

*3.2.17 Ọmụmụ ihe nhazi chromosome: A na-eji usoro PCR ezigbo oge iji nyochaa nhazi nke chromatin site n'ịtụle mmetụta nke nucleases na mmebi DNA genomic.Nke a na-egosipụta n'ezie njikọ dị elu n'etiti usoro chromatin na okwu mkpụrụ ndụ ihe nketa

4 Ngwa ndị dị mkpa

Ngwanrọ nyocha kọmputa na njikwa njikwa (gụnyere ọnụọgụ zuru oke, ọnụọgụ ikwu, nyocha mgbaze, nyocha njedebe, ntụnyere data ọtụtụ bọọdụ, wdg.)

5 Ogo nkwa oge

Oge nkwenye dị mma ga-abụ otu afọ mgbe ntinye na ntinye aka nabata ma nabata onye ọrụ.